ABSTRACT
The objective of this study was to develop Aceclofenac fast dispersible compacted pellets with improved taste and fast drug release. Pellets were prepared by extrusion-spheronization technique followed by direct compression to make compacted pellets. Formulations were comprised of sucrose, mannitol, ac-di-sol, aspartame, pine apple flavor and magnesium stearate. A mixture of distilled water and isopropyl alcohol [1:1] was used for wet massing. The effect of acdi-sol on the drug release pattern was examined and dissolution profile comparison was established. All formulations followed First order and Weibull models and f2 values indicated dissimilarity with the marketed immediate release product. Taste of compacted pellets was evaluated by a panel of 12 human volunteers. Formulation P5 was found to be an optimized formulation due to satisfactory quality attributes
ABSTRACT
A high-pressure liquid chromatography [HPLC-UV] based simple and specific method for simultaneous quantitative determination of aspirin, amlodipine besylate and simvastatin in a capsule formulation has been developed and validated according to ICH guidelines
Chromatographic separation of the three drugs was carried out by aSpherisorbODS2 reverse phase column [4.6 x 250 mm; 5 microm] using amobile phase, which consisted of 70: 30 [v/v] mixture of acetonitrile and triethylamine phosphate buffer [pH 3; 0.015 M] with final pH adjusted to 2.5 using dilute ortho-phosphoric acid, at a flow rate of ImL/min. The eluents were detected at UV wavelength of 237 nm and the retention times for aspirin, amlodipine besylate and simvastatin were -2.7 mins, -6.1 mins and 10.5mins, respectively. This method is suitable and specific for the three drugs and was found to be linear [R[2]>0.995], accurate, specific, reproducible and robust in the concentration range of 375 to 1125mcg/ml for aspirin, 25 to 75mcg/ml for amlodipine besylate and 50 to 150mcg/ml for simvastatin. This simple and convenient method could be easily utilized for the characterization and quantitation of the three drugs in a single formulation for combination therapy of cardiovascular diseases
ABSTRACT
The aim of this study is to evaluate the susceptibility and resistance pattern of clinical isolates causing different types of infections and to compare the efficacy of antibiotics namely Levofloxacin and Cefepime. The in-vitro antibacterial activity and resistance patterns of these two well known antibiotics were studied and compared using disk diffusion method. For this, one hundred clinical isolates comprising of Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae and Pseudomonas aeruginosa were collected from different local pathological laboratories and hospitals. Escherichia coli [17.95% against cefepime and 30.77% against levofloxacin], Staphylococcus aureus [30% against cefepime and 46.66% against levofloxacin] and Pseudomonas aeruginosa [23.53% against cefepime and 35.29% against levofloxacin] were found resistant against the studied antibiotics which show that cefepime is more effective than levofloxacin. In case of Klebsiella pneumoniae, resistance was 42.85% against cefepime and 35.71% against levofloxacin thereby showing that levofloxacin is more effective than cefepime. Concluded that the clinical isolates collected were susceptible to both the antibiotics but the microbial resistance against these antibiotics is increasing in our population which is alarming. Therefore, it is recommended the physicians may prescribe these antibiotics unless no other substitute is available in clinical practice